Synthesis of bioglass
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Bioactive glasses have been synthesized in many ways including conventional melting, quenching, the sol–gel process, flame synthesis and microwave irradiation. Bioglass synthesis has been reviewed by various groups, but one of the most frequently used methods is the sol-gel synthesis of bioglass composites, which is a highly efficient technique for tissue engineering applications.
Melt quench synthesis
The first bioactive glass, developed by Larry Hench in 1969, was produced by melting a mixture of related oxide precursors at relatively high temperatures. This original bioactive glass, named Bioglass, was melt-derived with a composition of 46.1 mol% SiO2, 24.4 mol% Na2O, 26.9 mol% CaO, and 2.6 mol% P2O5. The selection of the glass composition for specific applications should be based on comprehensive understanding of how each major component influences the properties of the glass, considering both its final use and its manufacturing process. Despite extensive research over the past 40 years, only a limited number of glass compositions have been approved for clinical use. Among these, the two melt-derived compositions approved by the U.S. Food and Drug Administration (FDA)—45S5 and S53P4—consist of the four oxides: SiO2, Na2O, CaO, and P2O5.[1][2] In general, a great number of elements can be dissolved in glasses. The effect of Al2O3, B2O3, Fe2O3, MgO,[3] SrO,[4] BaO, ZnO, Li2O, K2O, CaF2[5] and TiO2 on the in vitro or in vivo properties of certain compositions of bioactive glasses has been reported.[6][7][8][9][10][excessive citations] However, the influence of the composition on the properties and compatibility of bioactive and biodegradable glasses is not fully understood.
The scaffolds fabricated by melt quench technique have much less porosity which causes healing and defects in tissue integration during in-vivo testing.
Sol–gel process
The sol–gel process has a long history of use for synthesis of silicate systems and other oxides and it has become a widely spread research field with high technological relevance, such as for the fabrication of thin films, coatings, nanoparticles and fibers. Sol-gel processing technology at low temperatures, an alternative to traditional melt processing of glasses, involves the synthesis of a solution (sol), typically composed of metal-organic and metal salt precursors followed by the formation of a gel by chemical reaction or aggregation, and lastly thermal treatment for drying, organic removal, and sometimes crystallization and cooling treatment. The synthesis of specific silicate bioactive glasses by the sol–gel technique at low temperatures using metal alkoxides as precursors was shown in 1991 by Li et al.[11] For the synthesis of bioactive glasses, typical precursors used are tetraethyl orthosilicate, calcium nitrate and triethyl phosphate. After hydrolysis and poly-condensation reactions a gel is formed which subsequently is calcined at 600–700°C to form the glass. Based on the preparation method, sol–gel derived products, e.g. thin films or particles are highly porous exhibiting a high specific surface area. Recent work on fabricating bioactive silicate glass nanoparticles by sol–gel process has been carried out by Hong et al.[12] In their research, nanoscale bioactive glass particles were obtained by the combination of two steps; sol–gel route and co-precipitation method, wherein the mixture of precursors was hydrolyzed in acidic environment and condensed in alkaline condition separately, and then followed by a freeze-drying process. The morphology and size of bioactive glass nanoparticles could be tailored by varying the production conditions and the feeding ratio of reagents.
Different ions can be added to bioactive glasses, such as zinc, magnesium, zirconium, titanium, boron and silver in order to improve the glass functionality and bioactivity. However, it is usually difficult to synthesize bioactive glasses in nano-size scale with addition of those ions. More recently, Delben et al. have developed sol–gel-derived bioactive glass doped with silver and reported that the Si–O–Si bond number increased with increasing silver concentration and this resulted in structural densification.[13] It was also observed that quartz and metallic silver crystallization increased with the increase in silver content in bioactive glass while hydroxyapatite crystallization decreased.
There is wide agreement about the versatility of the sol–gel technique to synthesize inorganic materials and it has been shown to be suitable for production of a variety of bioactive glasses. However, the method is also limited in terms of compositions that can be produced. Moreover, remaining water or residual solvent content may result in complications of the method for the intended biomedical applications of the nanoparticles or nanofibers produced. Usually a high temperature calcination step is required to eliminate organics remnants. In addition, sol–gel processing is relatively time consuming and since it is not a continuous process, batch-to-batch variations may occur.[citation needed]
Newer methods
Newer methods including flame and microwave synthesis of bioglass have been gaining attention in recent years. Flame synthesis works by baking the powders directly in a flame reactor.[14] Microwave synthesis is a rapid and low-cost powder synthesis method in which precursors are dissolved in water, transferred to an ultrasonic bath, and irradiated.[15]
References
- ^ Hench, L.L. & Paschall, H.A. (1973) Direct chemical bond of bioactive glass-ceramic materials to bone and muscle, J Biomed Mater Res, Vol. 7, No. 3, pp. 25-42.
- ^ Andersson, O.H., Karlsson, K.H., Kangasniemi, K. & Xli-Urpo, A. (1988). Models for physical properties and bioactivity of phosphate opal glasses. Glastechnische Berichte, 61(10):300-305.
- ^ Watts SJ, Hill RG, O’Donnell MD, Law RV. Influence of magnesia on the structure and properties of bioactive glasses. J Non-Cryst Solids 2010;356:517-24.
- ^ Gentleman, E., Fredholm, Y.C., Jell, G., Lotfibakhshaiesh, N., O'Donnell, M.D., Hill, R.G. & Stevens, M.M. (2010) 'The effects of strontium-substituted bioactive glasses on osteoblasts and osteoclasts in vitro', Biomaterials, 31(14): 3949-3956.
- ^ V. Aina, G. Lusvardi, G. Malavasi, L. Menabue, C. Morterra, Fluoride-containing bioactive glasses: surface reactivity in simulated body fluids, Acta Biomaterialia 5 (2009) 3548–3562.
- ^ Andersson, Ö.H., Liu, G., Karlsson, K.H., Niemi, L., Miettinen, J. & Juhanoja, J. (1990) 'In vivo behaviour of glasses in the SiO2-Na2O-CaO-P2O5-Al2O3-B2O3 system', Journal of Materials Science: Materials in Medicine, 1(4): 219-227.
- ^ W.C.A. Vrouwenvelder, C.G. Groot, K. Degroot, Better histology and biochemistry for osteoblasts cultured on titanium doped bioactive glass — Bioglass 45S5 compared with iron-containing, titanium-containing, fluorine containing and boron-containing bioactive glasses, Biomaterials 15 (1994) 97–106.
- ^ Brink M, Turunen T, Happonen R-P, Yli-Urpo A. Compositional dependence of bioactivity of glasses in the system Na2O-K2O-MgO-CaO-B2O3-P2O5-SiO2. J Biomed Mater Res 1997;37:114-121.
- ^ Haimi, S., Gorianc, G., Moimas, L., Lindroos, B., Huhtala, H., Räty, S., Kuokkanen, H., Sándor, G.K., Schmid, C., Miettinen, S. & Suuronen, R. (2009) 'Characterization of zinc-releasing three Dimensional bioactive glass scaffolds and their effect on human adipose stem cell proliferation and osteogenic differentiation', Acta Biomaterialia, Vol. 5, No. 8, pp. 3122-3131.
- ^ Zhang, J., Wang, M., Cha, JM. & Mantalaris, A. (2009). The incorporation of 70s bioactive glass to the osteogenic differentiation of murine embryonic stems cells in 3D bioreactors. J. Tissue Eng. Regen. Med. 3(1): 63-71.
- ^ Li R, Clark AE, Hench LL. An Investigation of Bioactive Glass Powders by Sol- Gel Processing. J App Biomater 1991;2(4):231-239.
- ^ Hong Z, Liu A, Chen L, Chen X, Jing X. Preparation of bioactive glass ceramic nanoparticles by combination of sol-gel and coprecipitation method. J Non- Cryst Solids 2009;355(6):368-372
- ^ Delben JRJ, Pimentel OM, Coelho MB, Candelorio PD, Furini LN, Santos FA, Vicente FS, Delben AAST. Synthesis and thermal properties of nanoparticles of bioactive glasses containing silver. J Therm Anal Calorim 2009;97:433–436.
- ^ Brunner, Tobias J.; Grass, Robert N.; Stark, Wendelin J. (2006). "Glass and bioglass nanopowders by flame synthesis". Chemical Communications (13): 1384–6. doi:10.1039/b517501a. PMID 16550274.
- ^ ESSIEN, ENOBONG R; ATASIE, VIOLETTE N; UDOBANG, ESTHER U (27 July 2016). "Microwave energy-assisted formation of bioactive CaO–MgO–SiO2 ternary glass from bio-wastes" (PDF). Bulletin of Materials Science. 39 (4): 989–995. doi:10.1007/s12034-016-1251-6. S2CID 100064762.