English: FIGURE 1. Pedigree, sequencing electropherograms, conservation analysis, and clinical imaging features of distal hereditary motor neuropathy (dHMN) families with GARS mutation. (A) Pedigrees of family1 (GARS c. 373G>C) and family 2 (GARS c.1015G>A), and chromatograms of the mutation sites confirmed by Sanger sequencing. The GARS c.1015G>A variant is shown to be a de novo variant by comparing 22 core STR markers. Square, male; Circle, female; Black filled symbol, clinically and electromyogram confirmed affected individual; Empty symbol, clinically healthy individual; M, mutant type; W, wild-type; Arrows, probands. (B) Conservation of the residues surrounding Glu acid (E) 125 (red) and Gly (G) 339 in GARS among species. (C) Images of the upper and lower limbs of the proband in family 2(II-1).